LAG-3 Single Domain Antibody [1A6]

Product Specifications

Background

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2) . It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4) . LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

NCBI Gene ID

3902

Swiss Prot

P18627

Accession Number

NP_002277

Host

Llama

Reactivity

Human

Immunogen

LAG3 antibody was raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.

Clonality

Recombinant Monoclonal

Clone

1A6

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.

Concentration

1 mg/mL

Buffer

LAG-3 Antibody is supplied in PBS.

Modification

None

Shipping Conditions

Dry Ice

Storage Conditions

LAG3 antibody should be stored in working aliquots at -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

SdAb

Applications Notes

LAG-3 antibody can be used for ELISA starting at 0.1 μ g/mL. For immunohistochemistry starting at 1 μ g/mL. For immunofluorescence start at 5 μ g/mL. For flow cytometry at 1 μ g/ml.

Symbol

LAG3

NCBI Official Name

Lymphocyte activating 3

NCBI Organism

Homo sapiens

Background Reference 01

Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.