CTLA4 Antibody [1E6]

Product Specifications

Background

The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2) . Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3) . Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4) . CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5) .

NCBI Gene ID

1493

Swiss Prot

P16410

Accession Number

NP_005205

Host

Mouse

Reactivity

Human

Immunogen

CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.

Clonality

Monoclonal

Clone

[1E6]

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

CTLA-4 Antibody is supplied as protein A purified IgG1.

Positive Control

Cat. No. 1210 - HEK293 Lysate Cell Lysate

Concentration

1 mg/mL

Buffer

CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

CTLA-4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG1

Symbol

CTLA4

NCBI Official Name

Cytotoxic T-lymphocyte associated protein 4

NCBI Organism

Homo sapiens

Background Reference 01

Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--CTLA-4 . Nature 1987; 328:267–70.

Background Reference 02

Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1:405–13.

Background Reference 03

Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607–9.

Background Reference 04

Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.