PD1 Antibody [7H6] (biotin)

Product Specifications

Background

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.

NCBI Gene ID

5133

Swiss Prot

Q15116

Accession Number

NP_005009

Host

Mouse

Reactivity

Human

Immunogen

PD-1 antibody (biotin) was raised against the extracellular domain of human PD-1.

Clonality

Monoclonal

Clone

7H6

Conjugation

Biotin

Type

Primary Antibodies

Field of Research

Immunology

Purification

PD-1 Antibody (biotin) is supplied as protein A purified IgG1.

Positive Control

Cat. No. 1306 - Human Spleen Tissue Lysate

Concentration

1 mg/mL

Buffer

PD-1 Antibody (biotin) is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PD-1 Antibody (biotin) can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG1

Applications Notes

PD1 antibody can be used for as detection antibody in Sandwich Elisa at 1 μ g/mL.

Symbol

PDCD1

Positive Control 2

Cat. No. 1315 - Human Tonsil Tissue Lysate

Positive Control 3

Cat. No. 1369 - Human Lymph node Tissue Lysate

Positive Control 4

Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 5

Cat. No. 10-001 - Human Breast Tissue Slide

Positive Control 6

Cat. No. 17-010 - HEK293 Cell Slide

NCBI Official Name

Programmed cell death 1

NCBI Organism

Homo sapiens

Background Reference 01

Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.

Background Reference 02

Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.

Background Reference 03

Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.

Background Reference 04

Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.