DDDDK Antibody [1D1B12] (biotin)

Product Specifications

Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. DDDDK-tag is one of the more popular epitope tags for expressed recombinant proteins.

NCBI Gene ID

Swiss Prot

P60709

Host

Mouse

Reactivity

Tag

Immunogen

Biotin-DDDDK antibody was raised against a synthetic peptide containing DYKDDDDK.

Clonality

Monoclonal

Clone

1D1B12

Conjugation

Biotin

Type

Primary Antibodies

Field of Research

Other

Purification

Biotin-DDDDK antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Buffer

Biotin-DDDDK antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Biotin-DDDDK antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

Biotin-DDDDK-tag antibody will detect carboxy-terminal DDDDK-tagged recombinant proteins.

Applications Notes

Biotin-DDDDK antibody can be used for detection of DDDDK by Western blot at 0.5 - 1 μ /ml.

Symbol

ACTB

NCBI Official Name

Actin, beta

NCBI Organism

Homo sapiens

Other Product Names

Biotin-DDDDK, DDDDK, FLAG, FLAGtag, flag-tag

Tested Applications

ELISA, WB

Physical Properties

Liquid

Available Sizes

Curated Selection

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