Alpha-Tubulin Antibody [2B11]

Product Specifications

Background

Alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1) . Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2) . Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3) . Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

NCBI Gene ID

7846

Accession Number

CAA25855

Host

Mouse

Reactivity

Human, Mouse, Rat, Rabbit, Zebrafish

Immunogen

Alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin

Clonality

Monoclonal

Clone

2B11

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

Alpha-Tubulin antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1303 - Human Brain Tissue Lysate

Concentration

1 mg/mL

Buffer

Alpha-Tubulin antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Alpha-Tubulin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

Alpha-Tubulin antibody is human, mouse, rat, rabbit, and zebrafish reactive.

Symbol

TUBA1A

NCBI Official Name

Tubulin, alpha 1a

NCBI Organism

Homo sapiens

Background Reference 01

McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.

Background Reference 02

Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53.

Background Reference 03

Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.

Other Product Names

Tubulin alpha-1A, TUBA1A, TUBA3, LIS3

Tested Applications

ELISA, WB

Protein ID

37492

Physical Properties

Liquid

Available Sizes

Curated Selection

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