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GAPDH Antibody [12D3H9]

Product Specifications

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1) . GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2) . GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

NCBI Gene ID

2597

Swiss Prot

P04406

Accession Number

NP_002037

Host

Mouse

Reactivity

Human, Mouse, Rat

Clonality

Monoclonal

Clone

12D3H9

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction, Neuroscience, Homeostasis

Purification

GAPDH antibody is Protein A purified.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (100%), Sheep: (93%), Bovine: (93%), Chicken: (93%), Rabbit: (93%)

Buffer

GAPDH antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

GAPDH antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=PM-7571

Fragment

IgG

Specificity

GAPDH antibody is human, mouse, and rat reactive.

Symbol

GAPDH

NCBI Official Name

Glyceraldehyde-3-phosphate dehydrogenase

NCBI Organism

Homo sapiens

Background Reference 01

Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.

Background Reference 02

Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.

Other Product Names

Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD

Tested Applications

ELISA, WB

Protein ID

7669492

Physical Properties

Liquid

Available Sizes

Curated Selection

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