GAPDH Antibody [12D3D5]

Product Specifications

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1) . GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2) . GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

NCBI Gene ID

2597

Swiss Prot

P04406

Accession Number

NP_002037

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.

Clonality

Monoclonal

Clone

12D3D5

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction, Neuroscience, Homeostasis

Purification

GAPDH antibody is Protein A purified.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/mL

Buffer

GAPDH antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

GAPDH antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

GAPDH antibody is human, mouse, and rat reactive.

Symbol

GAPDH

NCBI Official Name

Glyceraldehyde-3-phosphate dehydrogenase

NCBI Organism

Homo sapiens

Background Reference 01

Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.