Beta-Actin Antibody [10B7]

Product Specifications

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

NCBI Gene ID

Accession Number

AAH02409

Host

Mouse

Reactivity

Human, Mouse, Rat, Rabbit, Zebrafish, Chicken, Drosophila

Immunogen

B-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human b-actin.

Clonality

Monoclonal

Clone

10B7

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

Beta-Actin Antibody is Protein G purified.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/mL

Buffer

Beta-Actin Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Beta-Actin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

Beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.

Symbol

ACTB

NCBI Official Name

Actin, beta

NCBI Organism

Homo sapiens

Background Reference 01

Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.