ERGIC53 Monoclonal Antibody

Product Specifications

Background

ERGIC53 Monoclonal Antibody: ERGIC53, also known as lectin mannose-binding 1 (LMAN1) protein is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.

NCBI Gene ID

3998

Accession Number

NP_005561

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Mouse monoclonal ERGIC53 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERGIC53.

Clonality

Monoclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

ERGIC53 Monoclonal Antibody is Protein A purified.

Positive Control

Cat. No. 1209 - HL60 Cell Lysate

Concentration

1 mg/mL

Buffer

ERGIC53 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ERGIC53 Monoclonal antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Calculated Molecular Weight

Predicted: 56 kDa

Fragment

IgG1

Specificity

At least two isoforms of ERGIC53 are known to exist.

Applications Notes

ERGIC53 antibody can be used for detection of ERGIC53 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ g/mL.

Symbol

LMAN1

NCBI Official Name

Lectin, mannose-binding, 1

NCBI Organism

Homo sapiens

Background Reference 01

Schindler R, Itin C, Zerial M, et al. ERGIC-53, a membrane protein of the ER-Golgi intermediate compartment, carries an ER retention motif. Eur. J. Cell Biol. 1993; 61:1-9.

Background Reference 02

ERGIC-53 is a functional mannose-selective and calcium-dependent human homologue of leguminous lectins. Mol. Biol Cell 1996; 7:483-93

Background Reference 03

Zhang YC, Zhou Y, Yang CZ, et al. A review of ERGIC-53: its structure, functions, regulation and relations with diseases. Histol. Histopathol. 2009; 24:1193-204.

Other Product Names

ERGIC53 Monoclonal Antibody: Lectin mannose-binding 1, LMAN1, endoplasmic reticulum-golgi intermediate compartment protein 53, ERGIC-53, F5F8D, FMFD1, MCFD1, MR60, gp58