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HIV-1 p24 Antibody [8G9] (biotin)

Product Specifications

Background

HIV-1 p24 Monoclonal Antibody: The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core and the region between the two termed matrix (1) . The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein (2) . The p24 constitutes the major core component of the virus and shows high degree of sequence conservation among HIV isolates. The Gag p24 has been used as an integral part of multicomponent HIV-1 vaccines (3) .

NCBI Gene ID

155030

Swiss Prot

P04591

Accession Number

AAB50258

Host

Mouse

Reactivity

Virus

Immunogen

Mouse monoclonal HIV-1 p24 antibody was raised against a recombinant full-length HIV-1 p24 protein.

Clonality

Monoclonal

Clone

8G9

Conjugation

Biotin

Type

Primary Antibodies, Antibody Pairs

Field of Research

Infectious Disease

Purification

HIV-1 p24 Monoclonal Antibody is Protein A purified.

Positive Control

Cat. No. 95-153 - Recombinant HIV-1 p24

Concentration

1 mg/mL

Buffer

HIV-1 p24 Monoclonal Antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

HIV-1 p24 Monoclonal Antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=PM-6335-biotin

Calculated Molecular Weight

Predicted: 24, 41, 55 kDa

Fragment

IgG1

Specificity

By Western blot, anti-HIV-1 p24 antibody detects a ~24 kDa, a ~41 kDa, and a ~55 kDa protein, corresponding to HIV-1 p24 and to its precursors p41 and p55, respectively, in HIV-1 samples.

Applications Notes

PM-6335-biotin can be used for detection of p24 by Western blot or ELISA at 0.2 – 0.5μ g/mL.

Symbol

GAG

NCBI Official Name

Gag Pr55 (Gag)

NCBI Organism

Hiv-1

Background Reference 01

Goto T, Nakai M, and Ikuta K. The life-cycle of human immunodeficiency virus type 1. Micron 1998; 29:123-38.

Background Reference 02

Freed EO. HIV-1 gag proteins: diverse functions in the virus life cycle. Virology 1998; 251:1-15.

Background Reference 03

Flynn BJ, Kastenmuller K, Wille-Reece U, et al. Immunization with HIV Gag targeted to dendritic cells followed by recombinant New York vaccinia virus induces robust T-cell immunity in nonhuman primates. Proc. Natl. Acad. Sci. USA 2011; 108:7131–6.

Other Product Names

HIV-1 p24 Antibody [8G9] :

Tested Applications

ELISA, WB

Protein ID

327745

Physical Properties

Liquid

Available Sizes

Curated Selection

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