KLF4 Antibody [4E5C3]

Product Specifications

Background

KLF4 Monoclonal Antibody: KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.

NCBI Gene ID

9314

Swiss Prot

O43474

Accession Number

AAH30811

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

KLF4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human KLF4.

Clonality

Monoclonal

Clone

4E5C3

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

KLF4 Monoclonal Antibody is Protein A purified.

Positive Control

Cat. No. 1404 - Mouse Liver Tissue Lysate

Concentration

1 mg/mL

Buffer

KLF4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

KLF4 monoclonal antibody can be stored at -20˚ C, stable for one year.

Fragment

IgG1

Specificity

At least three isoforms of KLF4 are known to exist; this antibody will detect all three. KLF4 antibody will not cross-react with other Kruppel-like family members.

Symbol

KLF4

NCBI Official Name

Kruppel-like factor 4 (gut)

NCBI Organism

Homo sapiens

Background Reference 01

Evans PM, Zhang W, Chen X, et al. Kruppel-like factor 4 is acetylated by p300 and regulates gene transcription via modulation of histone acetylation. J. Bio. Chem. 2007; 282:33994-4002.

Background Reference 02

Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells 2003; 5:79-88.

Background Reference 03

Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 1263:663-76.