IRAK Antibody [8F1A7]

Product Specifications

Background

IRAK Monoclonal Antibody: Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κ B activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) .

NCBI Gene ID

3654

Swiss Prot

P51617

Accession Number

P51617

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Recombinant protein corresponding to amino acids 618 - 712 of human IRAK.

Clonality

Monoclonal

Clone

8F1A7

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction, Innate Immunity

Purification

IRAK Monoclonal Antibody is Protein G purified.

Positive Control

Cat. No. 95-112 - IRAK Recombinant Protein

Concentration

1 mg/mL

Buffer

IRAK Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

IRAK monoclonal antibody can be stored at -20˚ C, stable for one year.

Fragment

IgG1

Symbol

IRAK1

NCBI Official Name

Interleukin-1 receptor-associated kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Cao Z, ; Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.

Background Reference 02

Trofimova M, Sprenkle AB, Green M; et al. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J. Bio. Chem. 1996; 271: 17609-12.

Background Reference 03

Jianing Huang, Xiong Gao, Shyun Li, et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc. Natl. Acad. Sci. USA 1997; 94:12829-32.

Background Reference 04

Robinson D, Shibuya K, Mui A, et al. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF-kB. Immunity 1997; 7:571-81.