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ORAI1 Antibody [3F6H5]

Product Specifications

Background

ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

NCBI Gene ID

84876

Swiss Prot

Q96D31

Accession Number

Q96D31

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.

Clonality

Monoclonal

Clone

3F6H5

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

ORAI1 Monoclonal Antibody is Protein A purified.

Positive Control

Cat. No. 1316 - Human Ovary Tissue Lysate

Concentration

1 mg/mL

Buffer

ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ORAI1 monoclonal antibody can be stored at -20˚ C, stable for one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=PM-5205

Fragment

IgG1

Specificity

ORAI1 antibody is predicted to have no cross reactivity to other members in the ORAI family.

Symbol

ORAI1

Positive Control 2

Cat. No. 10-901 - Human Spleen Tissue Slide

NCBI Official Name

ORAI calcium release-activated calcium modulator 1

NCBI Organism

Homo sapiens

Background Reference 01

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.

Background Reference 02

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

Background Reference 03

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.

Other Product Names

ORAI1 Antibody [3F6H5] : IMD9, ORAT1, CRACM1, TMEM142A

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

97180269

Physical Properties

Liquid

Available Sizes

Curated Selection

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