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CD4 Antibody [9H5A8]

Product Specifications

Background

CD4 Monoclonal Antibody: CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.

NCBI Gene ID

920

Swiss Prot

P01730

Accession Number

NP_000607

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.

Clonality

Monoclonal

Clone

9H5A8

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Recombinant Protein Test (Figure 1) : Anti-CD4 antibodies (PM-5201) detected human CD-4 recombinant protein at 5ng. Recombinant Protein TestPM-5201

Field of Research

Immunology

Purification

CD4 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Positive Control

Cat. No. 1314 - Human Thymus Tissue Lysate

Concentration

1 mg/mL

Buffer

CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

CD4 monoclonal antibody can be stored at -20˚ C, stable for one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=PM-5201

Fragment

IgG1

Isoform

Human CD4 has one isoform (458aa, 51kD) . Mouse CD4 has two isoforms, including isoform 1 (457aa, 51kD) and isoform 2 (217aa, 24.5kD) . Rat CD4 has one isoform (457aa, 51kD) . PM-5201 can detect human, mouse and rat.

Symbol

CD4

Positive Control 2

Cat. No. 1205 - Jurkat Cell Lysate

NCBI Official Name

CD4 molecule

NCBI Organism

Homo sapiens

Background Reference 01

Bowers et al. Int. J. Biochem. Cell Biol. 1997; 29 :871-5.

Background Reference 02

Arthos et al. Cell 1989; 57:469-81.

Citation 01

22954649

Other Product Names

CD4 Antibody [9H5A8] : CD4mut

Tested Applications

ELISA, WB, IHC-P

Protein ID

10835167

Physical Properties

Liquid

Available Sizes

Curated Selection

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