ORAI3 Antibody [2H2G9]

Product Specifications

Background

ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.

NCBI Gene ID

93129

Swiss Prot

Q9BRQ5

Accession Number

Q9BRQ5

Host

Mouse

Reactivity

Human, Rat

Immunogen

A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.

Clonality

Monoclonal

Clone

2H2G9

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

ORAI3 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Positive Control

Cat. No. 1466 - Rat Spleen Tissue Lysate

Concentration

1 mg/mL

Buffer

ORAI3 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ORAI3 monoclonal antibody can be stored at -20˚ C, stable for one year.

Fragment

IgG2a

Symbol

ORAI3

NCBI Official Name

ORAI calcium release-activated calcium modulator 3

NCBI Organism

Homo sapiens

Background Reference 01

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.

Background Reference 02

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

Background Reference 03

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.

Background Reference 04

Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.