MyD88 Antibody [2E9C2]

Product Specifications

Background

MyD88 Monoclonal Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κ B activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

NCBI Gene ID

4615

Swiss Prot

Q99836

Accession Number

AAC50954

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Mouse monoclonal MyD88 antibody was raised against a recombinant protein corresponding to amino acids 176 to 280 of human MyD88.

Clonality

Monoclonal

Clone

2E9C2

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity, Inflammasomes

Purification

MyD88 Monoclonal Antibody is Protein A purified.

Positive Control

Cat. No. 1287 - EL4 Cell Lysate

Concentration

1 mg/mL

Buffer

MyD88 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

MyD88 monoclonal antibody can be stored at -20˚ C, stable for one year.

Fragment

IgG2b

Symbol

MYD88

Positive Control 2

Cat. No. 95-104 - MyD88 Recombinant Protein

Positive Control 3

Cat. No. 10-501 - Human Heart Tissue Slide

NCBI Official Name

Myeloid differentiation primary response gene (88)

NCBI Organism

Homo sapiens

Background Reference 01

Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.

Background Reference 02

Adachi O, Kawai T, Takeda K, et al. Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function. Immunity 1998; 9:143-50.

Background Reference 03

Medzhitov R, Preston-Hurlburt P, Kopp E, et al. MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways. Mol. Cell 1998; 2:253-8.