Hemagglutinin Antibody [4E10C10]

Product Specifications

Background

Hemagglutinin Monoclonal Antibody: Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals, but it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with host cell surface proteins containing oligosaccharides with terminal sialyl residues. Its extracellular region has two domains (HA1 and HA2) ; HA1 is cleaved from the main hemagglutinin protein by the host immune system. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong. This more recent virulent strain of H5N1 is now seen in Africa and Europe, as well as in Southeast Asia. There is some evidence of human to human spread of this virus, but it is thought that the efficiency of this type of transmission is low. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability.

Swiss Prot

Q692M2

Accession Number

AAT76166

Host

Mouse

Reactivity

Virus

Immunogen

Recombinant protein corresponding to amino acids 17 - 338 of H5 HA1.

Clonality

Monoclonal

Clone

4E10C10

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Infectious Disease

Purification

Hemagglutinin Monoclonal Antibody is Protein A purified.

Positive Control

Cat. No. 95-101 - H5 Hemagglutinin HA1 Recombinant Protein

Concentration

1 mg/mL

Buffer

Hemagglutinin Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Hemagglutinin monoclonal antibody can be stored at -20˚ C, stable for one year.

Fragment

IgG1, IgG2b

Applications Notes

Hemagglutinin antibody can be used for detection of H5 HA1 by Western blot at 2.5 μ g/mL. Hemagglutinin antibody also specifically recognizes Avian H5N1 influenza virus at 2 μ g/mL.

NCBI Official Name

Hemagglutinin

NCBI Organism

Influenza A virus (A/chicken/Jilin/9/2004 (H5N1) )

Background Reference 01

Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003; 289:179-186.

Background Reference 02

Alexander DJ. A review of avian influenza. Proceedings of the European Society for Veterinary Virology (ESVV) Symposium on Influenza Viruses of Wild and Domestic Animals. Vet. Microbiol. 2000; 74:3-13.

Background Reference 03

Shortridge KF, Zhou NN, Guan Y, et al. Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virol. 1998; 252:331-342.

Background Reference 04

Buxton Bridges C, Katz JM, Seto WH, et al. Risk of influenza A (H5N1) infection among health care workers exposed to patients with influenza A (H5N1), Hong Kong. J. Inf. Dis. 2000; 181:344-8.