PUMA Antibody [10D4G7]

Product Specifications

Background

PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ . PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

NCBI Gene ID

27113

Swiss Prot

Q9BXH1

Accession Number

Q9BXH1

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.

Clonality

Monoclonal

Clone

10D4G7

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis

Purification

PUMA Monoclonal Antibody is Protein A purified.

Positive Control

Cat. No. 1204 - K562 Cell Lysate

Concentration

1 mg/mL

Buffer

PUMA Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PUMA monoclonal antibody can be stored at -20˚ C, stable for one year.

Fragment

IgG1

Symbol

BBC3

Positive Control 2

Cat. No. 95-102 - PUMA Recombinant Protein

NCBI Official Name

BCL2 binding component 3

NCBI Organism

Homo sapiens

Background Reference 01

Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.

Background Reference 02

Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.

Background Reference 03

Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.