Apaf-1 Antibody [2E10]

Product Specifications

Background

Apaf-1 Monoclonal Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.

NCBI Gene ID

317

Swiss Prot

O14727

Accession Number

AAC51678

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.

Clonality

Monoclonal

Clone

2E10

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis

Purification

Apaf-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Positive Control

Cat. No. 1204 - K562 Cell Lysate

Concentration

1 mg/mL

Buffer

Apaf-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Apaf-1 monoclonal antibody can be stored at -20˚ C, stable for one year.

Calculated Molecular Weight

130 kDa

Fragment

IgG1

Symbol

APAF1

Positive Control 2

Cat. No. 17-004 - K-562 Cell Slide

NCBI Official Name

Apoptotic peptidase activating factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Zou H, Henzel WJ, Liu X, et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997; 90:405-13.

Background Reference 02

Cecconi F, Alvarez-Bolado G, Meyer BI, et al. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998; 94:727-37.

Background Reference 03

Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.

Background Reference 04

Hu Y, Benedict MA, Wu D, et al. Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc. Natl. Acad. Sci. USA 1998; 95:4386-91.