CD8a Antibody [3-298]

In the chicken the CD8 molecule is present in two forms - (i) a homodimer of two alpha chains and (ii) a heterodimer of an alpha chain and a beta chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCRgammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRgammadelta CD8alphabeta cells in the chicken that is not found in rodents. The monoclonal antibody 3-298 recognizes the CD8alpha chain of both the chicken and turkey.