CD8a Antibody [53-6.7] (FITC)
Product Specifications
Background
In the mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an alpha chain (CD8alpha) and a beta chain (CD8beta) ; and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8alpha without CD8beta. It has been suggested that CD8+beta- T cells mature extrathymically, while development of the CD8alpha+beta+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8alpha+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
NCBI Gene ID
12525
Swiss Prot
P01731
Accession Number
P01731
Host
Rat
Reactivity
Mouse
Immunogen
Spleen cells or thymocyte membranes
Clonality
Monoclonal
Clone
[53-6.7]
Conjugation
FITC
Type
Primary Antibodies
Field of Research
Immunology
Concentration
0.5 mg/mL
Buffer
Supplied in PBS/NaN3
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store vial at 2-8˚ C
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=98-614
Fragment
IgG2a
Specificity
CD8alpha
Applications Notes
CD8a Antibody [53-6.7] for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
Symbol
Cd8a
NCBI Official Name
CD8 antigen, alpha chain
NCBI Organism
Mus musculus
Other Product Names
Ly-2, Lyt-2, Ly-35, Ly-B, T8
Tested Applications
ELISA, IHC-Fr, ICC
Physical Properties
Liquid
Curated Selection
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