Phosphotyrosine Antibody [PY20] (HRP)
Product Specifications
Background
Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate.
Host
Mouse
Immunogen
Phosphotyrosine conjugated to carrier proteins
Clonality
Monoclonal
Clone
[PY20]
Conjugation
HRP
Type
Primary Antibodies
Field of Research
Obesity, Signal Transduction, Neuroscience, Cancer
Concentration
Batch dependent
Buffer
Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store vial at 2-8˚ C
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=98-516
Fragment
IgG2b
Specificity
Phosphotyrosine
Applications Notes
Phosphotyrosine Antibody [PY20] for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.
Other Product Names
PTyr20
Tested Applications
ELISA
Physical Properties
Liquid
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