PECAM-1 (phospho Tyr713) Antibody

Product Specifications

Background

Induces susceptibility to atherosclerosis By similarity. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte.

NCBI Gene ID

5175

Swiss Prot

P16284

Accession Number

NP_000433.4

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

PECAM-1 (phospho Tyr713) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine 713 (T-V-Y (p) -S-E) derived from Human PECAM-1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

82, 150 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of PECAM-1 only when phosphorylated at tyrosine 713.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

PECAM1

NCBI Official Name

Platelet/endothelial cell adhesion molecule 1

NCBI Organism

Homo sapiens

Background Reference 01

Garnacho C, et al. (2008) Blood 111, 3024-33. Udell CM, et al. (2006) J Biol Chem 281, 20949-57 . O'Brien CD, Cao G, Makrigiannakis A, DeLisser HM (2004) Am J Physiol Cell Physiol 287, C1103-13.