AurB (phospho Thr232) Antibody

Product Specifications

Background

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity.

NCBI Gene ID

9212

Swiss Prot

Q96GD4

Accession Number

NP_001243763.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

AurB (phospho Thr232) antibody was raised against a peptide sequence around phosphorylation site of threonine 232 (R-K-T (p) -M-C) derived from Human AurB.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

39 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of AurB only when phosphorylated at threonine 232.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

AURKB

NCBI Official Name

Aurora kinase B

NCBI Organism

Homo sapiens

Background Reference 01

Caldas GV, DeLuca KF, DeLuca JG (2013) J Cell Biol 203, 957-69. Zhang L, et al. (2012) J Biol Chem 287, 34069-77. Ratushny V, et al. (2012) Oncogene 31, 1217-27 .