CaMK4 (phospho Thr196/200) Antibody

Product Specifications

Background

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2) .

NCBI Gene ID

814

Swiss Prot

Q16566

Accession Number

NP_001735.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

CaMK4 (phospho Thr196/200) antibody was raised against a peptide sequence around phosphorylation site of threonine 200 (M-K-T (p) -V-C) derived from Human CaMK4 or threonine 196 (M-K-T (p) -V-C) derived from Mouse CaMK4.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

60 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of CaMK4 only when phosphorylated at threonine196/threonine 200.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CAMK4

NCBI Official Name

Calcium/calmodulin-dependent protein kinase IV

NCBI Organism

Homo sapiens

Background Reference 01

Oury F, et al. (2010) Genes Dev 24, 2330-42. Dias WB, Cheung WD, Wang Z, Hart GW (2009) J Biol Chem 284, 21327-37. Chow FA, Anderson KA, Noeldner PK, Means AR (2005) J Biol Chem 280, 20530-8.