NMDAR1 (phospho Ser890) Antibody

Product Specifications

Background

NMDA receptors are members of the ionotropic class of glutamate receptors, which also includes Kainate and AMPA receptors. NMDA receptors consist of NR1 subunits combined with one or more NR2 (A-D) or NR3 (A-B) subunits. The ligand-gated channel is permeable to cations including Ca2+, and at resting membrane potentials NMDA receptors are inactive due to a voltage-dependent blockade of the channel pore by Mg2+. NMDA receptor activation, which requires binding of glutamate and glycine, leads to an influx of Ca2+ into the postsynaptic region where it activates several signaling cascades, including pathways leading to the induction of long-term potentiation (LTP) and depression (LTD) . NMDA receptors have a critical role in excitatory synaptic transmission and plasticity in the CNS. They govern a range of physiological conditions including neurological disorders caused by excitotoxic neuronal injury, psychiatric disorders and neuropathic pain syndromes.

NCBI Gene ID

2902

Swiss Prot

Q05586

Accession Number

NP_000823.4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

NMDAR1 (phospho Ser890) antibody was raised against a peptide sequence around phosphorylation site of serine890 (A-S-S (p) -F-K) derived from Human NMDAR1 .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

105 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of NMDAR1 only when phosphorylated at serine 890.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

GRIN1

NCBI Official Name

Glutamate receptor, ionotropic, N-methyl D-aspartate 1

NCBI Organism

Homo sapiens

Background Reference 01

Xu M, Smothers CT, Woodward JJ (2011) Alcohol 45, 373-80. Kong M, Ba M, Song L, Liu Z (2009) Neurochem Res 34, 1513-21 . Jackson MF, et al. (2006) J Neurochem 96, 1509-18.