C-Met (phospho Tyr1003) Antibody

Product Specifications

Background

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

NCBI Gene ID

4233

Swiss Prot

P08581

Accession Number

NP_000236.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

C-Met (phospho Tyr1003) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1003 (V-D-Y (p) -R-A) derived from Human c-Met.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

155 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of c-Met only when phosphorylated at tyrosine 1003.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

MET

NCBI Official Name

Met proto-oncogene

NCBI Organism

Homo sapiens

Background Reference 01

Usatyuk PV, et al. (2014) J Biol Chem. Asiedu MK, et al. (2014) Oncogene 33, 1316-24. Dulak AM, et al. (2011) Oncogene 30, 3625-35.

Other Product Names

HGF receptor, HGF-SF receptor, Hepatocyte growth factor receptor precursor, Hepatocyte growth factor receptor precursor, Met proto- oncogene tyros

Tested Applications

Protein ID

42741655

Physical Properties

Liquid

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