Pin1 (phospho Ser16) Antibody

Product Specifications

Background

Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner.

NCBI Gene ID

5300

Swiss Prot

Q13526

Accession Number

NP_006212.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Pin1 (phospho Ser16) antibody was raised against a peptide sequence around phosphorylation site of serine16 (R-M-S (p) -R-S) derived from Human Pin1 .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

18 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of Pin1 only when phosphorylated at serine 16.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

PIN1

NCBI Official Name

Peptidylprolyl cis/trans isomerase, NIMA-interacting 1

NCBI Organism

Homo sapiens

Background Reference 01

Lee YC, et al. (2013) J Cell Sci 126, 4862-72. Ando K, et al. (2013) Neurobiol Aging 34, 757-69. Nechama M, Lin CL, Richter JD (2013) Mol Cell Biol 33, 48-58.