CDC25B (phospho Ser353) Antibody

Product Specifications

Background

Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

NCBI Gene ID

994

Swiss Prot

P30305

Accession Number

NP_001274445.1

Host

Rabbit

Reactivity

Human

Immunogen

CDC25B (phospho Ser353) antibody was raised against a peptide sequence around phosphorylation site of serine 353 (R-R-S (p) -V-T) derived from Human CDC25B .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

64 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of CDC25B only when phosphorylated at serine 353.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CDC25B

NCBI Official Name

Cell division cycle 25B

NCBI Organism

Homo sapiens

Background Reference 01

Reboutier D, et al. (2012) J Cell Biol 197, 19-26

Background Reference 02

Lobjois V, et al. (2011) Biochem Biophys Res Commun 410, 87-90

Background Reference 03

Feng C, et al. (2007) Biol Reprod 77, 560-8

Other Product Names

CDC25M2, MPIP2, Dual specificity phosphatase Cdc25B, CDC25HU2

Tested Applications

Protein ID

11641413

Physical Properties

Liquid

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