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ATPase (phospho Ser16) Antibody

Product Specifications

Background

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

NCBI Gene ID

476

Swiss Prot

P05023

Accession Number

NP_000692.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

ATPase (phospho Ser16) antibody was raised against a peptide sequence around phosphorylation site of serine 16 (A-V-S (p) -E-Q) derived from Human ATPase.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-988

Calculated Molecular Weight

112 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of ATPase only when phosphorylated at serine 16.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

ATP1A1

NCBI Official Name

ATPase, Na+/K+ transporting, alpha 1 polypeptide

NCBI Organism

Homo sapiens

Background Reference 01

Kava L, Rossi NF, Mattingly R, Yingst DR (2012) Am J Hypertens 25, 487-91

Background Reference 02

Soltoff SP, Asara JM, Hedden L (2010) J Biol Chem 285, 36330-8

Background Reference 03

Efendiev R, et al. (2006) FEBS Lett 580, 5067-70

Other Product Names

A1A1, AT1A1, ATP1A1, K-ATPase 1, Sodium pump 1

Tested Applications

WB

Protein ID

114374

Physical Properties

Liquid

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