IKK- alpha/ beta (phospho Ser176/177) Antibody

Product Specifications

Background

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3.

NCBI Gene ID

1147

Swiss Prot

O15111; O14920

Accession Number

NP_001269.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

IKK- alpha/ beta (phospho Ser176/177) antibody was raised against a peptide sequence around phosphorylation site of serine 176/177 (Q-G-S (p) -L-C) derived from Human IKK-alpha/beta.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

85 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 176/177.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CHUK

NCBI Official Name

Conserved helix-loop-helix ubiquitous kinase

NCBI Organism

Homo sapiens

Background Reference 01

Chandrakesan P, et al. (2010) J Biol Chem 285, 33485-98

Background Reference 02

Hinz M, et al. (2010) Mol Cell 40, 63-74

Background Reference 03

Choudhary S, Lu M, Cui R, Brasier AR (2007) Mol Endocrinol 21, 2203-17

Other Product Names

FLJ40509, I-kappa-B kinase, IKBKB, kinase beta, NFKBIKB

Tested Applications

Protein ID

62241001

Physical Properties

Liquid

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