IKK-γ (phospho Ser85) Antibody

Product Specifications

Background

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.

NCBI Gene ID

8517

Swiss Prot

Q9Y6K9

Accession Number

NP_001093326.2

Host

Rabbit

Reactivity

Human

Immunogen

IKK-γ (phospho Ser85) antibody was raised against a peptide sequence around phosphorylation site of serine 85 (Q-A-S (p) -Q-R) derived from Human IKK-gamma.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

48 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of IKK-γ only when phosphorylated at serine 85.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

IKBKG

NCBI Official Name

Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma

NCBI Organism

Homo sapiens

Background Reference 01

Niu J, Shi Y, Iwai K, Wu ZH (2011) EMBO J 30, 3741-53. Wu ZH, et al. (2010) Mol Cell 40, 75-86. Palkowitsch L, Leidner J, Ghosh S, Marienfeld RB (2008) J Biol Chem 283, 76-86.