SirT1 (phospho Ser47) Antibody

Product Specifications

Background

NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively.

NCBI Gene ID

23411

Swiss Prot

Q96EB6

Accession Number

NP_001135970.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

SirT1 (phospho Ser47) antibody was raised against a peptide sequence around phosphorylation site of serine 47 (E-R-S (p) -P-G) derived from Human SirT1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

110 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of SirT1 only when phosphorylated at serine 47.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

SIRT1

NCBI Official Name

Sirtuin 1

NCBI Organism

Homo sapiens

Background Reference 01

Gao Z, et al. (2011) J Biol Chem 286, 22227-34. Zschoernig B, Mahlknecht U (2009) Biochem Biophys Res Commun 381, 372-7. Nasrin N, et al. (2009) PLoS One 4, e8414.