Smad2 (phospho Ser250) Antibody

Product Specifications

Background

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD) . May act as a tumor suppressor in colorectal carcinoma.

NCBI Gene ID

4087

Swiss Prot

Q15796

Accession Number

NP_001003652.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Smad2 (phospho Ser250) antibody was raised against a peptide sequence around phosphorylation site of serine250 (E-L-S (p) -P-T) derived from Human Smad2 .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

65 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of Smad2 only when phosphorylated at serine 250.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

SMAD2

NCBI Official Name

SMAD family member 2

NCBI Organism

Homo sapiens

Background Reference 01

Fransvea E, Mazzocca A, Antonaci S, Giannelli G (2009) Hepatology 49, 839-50 . Funaba M, Zimmerman CM, Mathews LS (2002) J Biol Chem 277, 41361-8. Kretzschmar M, Doody J, Timokhina I, Massagué J (1999) Genes Dev 13, 804-16 .