FAK (phospho Ser843) Antibody

Product Specifications

Background

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

NCBI Gene ID

5747

Swiss Prot

Q05397

Accession Number

NP_001186578.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

FAK (phospho Ser843) antibody was raised against a peptide sequence around phosphorylation site of serine843 (R-G-S (p) -I-D) derived from Human FAK .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

120 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of FAK only when phosphorylated at serine 843.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

PTK2

NCBI Official Name

Protein tyrosine kinase 2

NCBI Organism

Homo sapiens

Background Reference 01

Jiang X, Sinnett-Smith J, Rozengurt E (2007) Cell Signal 19, 1000-10 . Jacamo R, Jiang X, Lunn JA, Rozengurt E (2007) J Cell Physiol 210, 436-44. Le Boeuf F, Houle F, Sussman M, Huot J (2006) Mol Biol Cell 17, 3508-20 .