Smad3 (phospho Ser204) Antibody

Product Specifications

Background

Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

NCBI Gene ID

4088

Swiss Prot

P84022

Accession Number

NP_001138574.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Smad3 (phospho Ser204) antibody was raised against a peptide sequence around phosphorylation site of serine204 (A-G-S (p) -P-N) derived from Human Smad3 .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

48 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of Smad3 only when phosphorylated at serine 204.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

SMAD3

NCBI Official Name

SMAD family member 3

NCBI Organism

Homo sapiens

Background Reference 01

Cohen-Solal KA, et al. (2011) Pigment Cell Melanoma Res 24, 512-24

Background Reference 02

Seong HA, Jung H, Ha H (2010) J Biol Chem 285, 30959-70

Background Reference 03

Zelivianski S, Cooley A, Kall R, Jeruss JS (2010) Mol Cancer Res 8, 1375-87

Other Product Names

JV15-2, MAD-3, MADH3, Smad 3, Mothers against decapentaplegic homolog 3

Tested Applications

Protein ID

223029440

Physical Properties

Liquid

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MBS7043432-01 0.02 mg

TGF-beta receptor type-2

Ask

View Details

MBS7043432-02 0.1 mg

TGF-beta receptor type-2

Ask

View Details

MBS7043432-03 5x 0.1 mg

TGF-beta receptor type-2

Ask

View Details

GTR15336256 1 Vial

Lentiviral human CHRM4 shRNA (UAS) - Lentiviral human CHRM4 shRNA (UAS, RFP) plasmid

Ask

View Details

38890111 3x 1.0 µg

RG9MTD2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

Ask

View Details

MR220969L4 10 µg

Ralgds (NM_001145835) Mouse Tagged Lenti ORF Clone

Ask

View Details