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Akt (phospho Ser129) Antibody

Product Specifications

Background

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

NCBI Gene ID

207

Swiss Prot

P31749

Accession Number

NP_001014431.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Akt (phospho Ser129) antibody was raised against a peptide sequence around phosphorylation site of serine129 (D-N-S (p) -G-A) derived from Human Akt .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

65 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of Akt only when phosphorylated at serine 129.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

AKT1

NCBI Official Name

V-akt murine thymoma viral oncogene homolog 1

NCBI Organism

Homo sapiens

Background Reference 01

Nguyen le XT, Mitchell BS (2013) Proc Natl Acad Sci U S A 110, 20681-6

Background Reference 02

Ponce DP, et al. (2011) Mol Cell Biochem 356, 127-32

Background Reference 03

Siddiqui-Jain A, et al. (2010) Cancer Res 70, 10288-98

Other Product Names

AKT, AKT1 kinase, kinase Akt1, RAC, RAC-PK-alpha

Tested Applications

WB

Protein ID

62241013

Physical Properties

Liquid

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