PKCB (phospho Ser661) Antibody

Product Specifications

Background

Calcium-activated, phospholipid- and diacylglycerol (DAG) -dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1.

NCBI Gene ID

5579

Swiss Prot

P05771

Accession Number

NP_002729.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PKCB (phospho Ser661) antibody was raised against a peptide sequence around phosphorylation site of serine 661 (G-F-S (p) -Y-T) derived from Human PKCB.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

77 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of PKCB only when phosphorylated at serine 661.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

PRKCB

NCBI Official Name

Protein kinase C, beta

NCBI Organism

Homo sapiens

Background Reference 01

Zhu M, Chen J, Jiang H, Miao C (2013) Cardiovasc Diabetol 12, 13

Background Reference 02

Leonard TA, et al. (2011) Cell 144, 55-66

Background Reference 03

Qiao M, et al. (2010) Mol Cell 38, 512-23

Other Product Names

PIM-1, EC 2.7.11.1

Tested Applications

Protein ID

20127450

Physical Properties

Liquid

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MF-0148207-01 100 mg

KG-655

Ask

View Details

MF-0148207-02 25 mg

KG-655

Ask

View Details

MF-0148207-03 50 mg

KG-655

Ask

View Details

abx657861-01 50 µg

Cow Pou Domain, Class 5, Transcription Factor 1 (POU5F1) Protein

Ask

View Details

abx657861-02 100 µg

Cow Pou Domain, Class 5, Transcription Factor 1 (POU5F1) Protein

Ask

View Details

abx657861-03 200 µg

Cow Pou Domain, Class 5, Transcription Factor 1 (POU5F1) Protein

Ask

View Details