BRCA2 (phospho Ser3291) Antibody

Product Specifications

Background

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA) . Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.

NCBI Gene ID

675

Swiss Prot

P51587

Accession Number

NP_000050.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

BRCA2 (phospho Ser3291) antibody was raised against a peptide sequence around phosphorylation site of Serine 3291 (F-V-S (p) -P-A) derived from Human BRCA2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

>350 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of BRCA2 only when phosphorylated at serine 3291.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

BRCA2

NCBI Official Name

Breast cancer 2, early onset

NCBI Organism

Homo sapiens

Background Reference 01

Wooster R., Nature 378:789-792 (1995) .

Background Reference 02

Tavtigian S.V., Nat. Genet. 12:333-337 (1996) .

Background Reference 03

Dunham A., Nature 428:522-528 (2004) .

Other Product Names

BRCC2, BRCA2, subunit 2, BRCA1/BRCA2-containing complex

Tested Applications

Protein ID

14424438

Physical Properties

Liquid

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