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Smad2 (phospho Ser465) Antibody

Product Specifications

Background

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD) . May act as a tumor suppressor in colorectal carcinoma.

NCBI Gene ID

4087

Swiss Prot

Q15796

Accession Number

NP_001003652.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Smad2 (phospho Ser465) antibody was raised against a peptide sequence around phosphorylation site of Serine 465 (C-S-S (p) -M-S) derived from Human Smad2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-947

Calculated Molecular Weight

52 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of Smad2 only when phosphorylated at serine 465.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

SMAD2

NCBI Official Name

SMAD family member 2

NCBI Organism

Homo sapiens

Background Reference 01

Riggins G.J., Nat. Genet. 13:347-349 (1996) .

Background Reference 02

Zhang Y., Nature 383:168-172 (1996) .

Background Reference 03

Eppert K., Cell 86:543-552 (1996) .

Other Product Names

HMAD-2, JV18, JV18-1, MAD, hSMAD2

Tested Applications

WB

Protein ID

51173730

Physical Properties

Liquid

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