EPHA4 (phospho Tyr596) Antibody

Product Specifications

Background

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity.

NCBI Gene ID

2043

Swiss Prot

P54764

Accession Number

NP_004429.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

EPHA4 (phospho Tyr596) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 596 (R-T-Y (p) -V-D) derived from Human EPHA4 .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

110 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of EPHA4 only when phosphorylated at tyrosine 596

Applications Notes

Western Blot: 1:500~1:1000

Symbol

EPHA4

NCBI Official Name

EPH receptor A4

NCBI Organism

Homo sapiens

Background Reference 01

Rikova K., Cell 131:1190-1203 (2007) [PubMed: 18083107].

Background Reference 02

Richter M., J. Neurosci. 27:14205-14215 (2007) [PubMed: 18094260].

Background Reference 03

Fu W.Y., Nat. Neurosci. 10:67-76 (2007) [PubMed: 17143272].

Other Product Names

EK8, hEK8, EPH-like kinase 8

Tested Applications

Protein ID

1711371

Physical Properties

Liquid

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