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MAPK1/3 (phospho Tyr205/222) Antibody

Product Specifications

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

NCBI Gene ID

5594;5595

Swiss Prot

P28482; P27361

Accession Number

NP_002736.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MAPK1/3 (phospho Tyr205/222) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 205/222 (K-G-Y (p) -T-K) derived from Human MAPK1/3 .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

44 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of MAPK1/3 only when phosphorylated at tyrosine 205/222.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

MAPK1, MAPK3

NCBI Official Name

Mitogen-activated protein kinase 1, mitogen-activated protein kinase 3

NCBI Organism

Homo sapiens

Background Reference 01

Owaki H., Biochem. Biophys. Res. Commun. 182:1416-1422 (1992) .

Background Reference 02

Dunham I., Nature 402:489-495 (1999) .

Other Product Names

ERK, ERT1, MAPK 1, MAPK2, ERK2

Tested Applications

WB

Protein ID

66932916

Physical Properties

Liquid

Frequently Asked Questions

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