HCK (phospho Tyr521) Antibody

Product Specifications

Background

The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

NCBI Gene ID

3055

Swiss Prot

P08631

Accession Number

NP_002101.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

HCK (phospho Tyr521) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 521 (E-S-Q (p) -Y-Q) derived from Human HCK .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

60 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of HCK only when phosphorylated at tyrosine 521.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

HCK

NCBI Official Name

HCK proto-oncogene, Src family tyrosine kinase

NCBI Organism

Homo sapiens

Background Reference 01

Quintrell N., Mol. Cell. Biol. 7:2267-2275 (1987) [PubMed: 3496523].

Background Reference 02

Ziegler S.F., Mol. Cell. Biol. 7:2276-2285 (1987) [PubMed: 3453117].

Background Reference 03

Ota T., Nat. Genet. 36:40-45 (2004) [PubMed: 14702039].

Other Product Names

P59-HCK/p60-HCK, Hemopoietic cell kinas, Tyrosine-protein kinase HCK

Tested Applications

Protein ID

287326139

Physical Properties

Liquid

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