FADD (phospho Ser191) Antibody

Product Specifications

Background

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

NCBI Gene ID

14082

Swiss Prot

Q13158

Accession Number

NP_003815.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

FADD (phospho Ser191) antibody was raised against a peptide sequence around phosphorylation site of Serine191 (R-S-G (p) -A-M) derived from Human FADD.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific , Inflammasomes

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

25 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of FADD only when phosphorylated at serine 191.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

FADD

NCBI Official Name

Fas (TNFRSF6) -associated via death domain

NCBI Organism

Homo sapiens

Background Reference 01

Sugano S., Nat. Genet. 36:40-45 (2004) .

Background Reference 02

Farmer A., Submitted (MAY-2003) .

Background Reference 03

Venter J.C., Submitted (JUL-2005) .

Other Product Names

MORT1 , FAS-associating death domain-containing protein, Mediator of receptor induced toxicity

Tested Applications

WB, IHC

Protein ID

6753812

Physical Properties

Liquid

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