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YB1 (phospho Ser102) Antibody

Product Specifications

Background

YB-1 is a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs) . Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A) n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP.

NCBI Gene ID

4904

Swiss Prot

P67809

Accession Number

NP_004550.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

YB1 (phospho Ser102) antibody was raised against a peptide sequence around phosphorylation site of Serine 102 (L-R-S (p) -V-G) derived from Human YB1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

36 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of YB1 only when phosphorylated at serine 102.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

YBX1

NCBI Official Name

Y box binding protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Sakura H., Gene 73:499-507 (1988) .

Background Reference 02

Didier D.K. Proc. Natl. Acad. Sci. U.S.A. 85:7322-7326 (1988) .

Background Reference 03

Kolluri R., Nucleic Acids Res. 19:4771-4771 (1991) .

Other Product Names

CBF-A, NSEP1, EFI-A, MSY-1, YBX1

Tested Applications

WB

Protein ID

34098946

Physical Properties

Liquid

Curated Selection

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