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PLD1 (phospho Thr147) Antibody

Product Specifications

Background

Phosphatidylcholine (PC) -specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis.

NCBI Gene ID

5337

Swiss Prot

Q13393

Accession Number

NP_002653.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PLD1 (phospho Thr147) antibody was raised against a peptide sequence around phosphorylation site of threonine 147 (R-H-T (p) -F-R) derived from Human PLD1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

120 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

PLD1

NCBI Official Name

Phospholipase D1, phosphatidylcholine-specific

NCBI Organism

Homo sapiens

Background Reference 01

Hammond S.M., J. Biol. Chem. 272:3860-3868 (1997) [PubMed: 9013646].

Background Reference 02

Steed P.M., FASEB J. 12:1309-1317 (1998) [PubMed: 9761774].

Background Reference 03

Lopez I., J. Biol. Chem. 273:12846-12852 (1998) [PubMed: 9582313].

Other Product Names

PLD 1, hPLD1, Choline phosphatase 1

Tested Applications

WB

Protein ID

4505873

Physical Properties

Liquid

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