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P47 phox (phospho Ser345) Antibody

Product Specifications

Background

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production) .

NCBI Gene ID

653361

Swiss Prot

P14598

Accession Number

NP_000256.4

Host

Rabbit

Reactivity

Human

Immunogen

P47 phox (phospho Ser345) antibody was raised against a peptide sequence around phosphorylation site of Serine 345 (P-Q-S (p) -P-G) derived from Human Nuclear p47 phox.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-920

Calculated Molecular Weight

45 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of p47 phox only when phosphorylated at serine 345.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

NCF1

NCBI Official Name

Neutrophil cytosolic factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199 (1989) .

Background Reference 02

Lomax K.J., Science 245:409-412 (1989) .

Background Reference 03

Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396 (1990) .

Other Product Names

NCF1, P47 phox, NCF-47K

Tested Applications

WB, IHC

Protein ID

115298672

Physical Properties

Liquid

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