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CAD (phospho Thr456) Antibody

Product Specifications

Background

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.

NCBI Gene ID

790

Swiss Prot

P27708

Accession Number

NP_004332.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

CAD (phospho Thr456) antibody was raised against a peptide sequence around phosphorylation site of threonine 456 (P-I-T (p) -P-H) derived from Human CAD.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

242 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of CAD only when phosphorylated at threonine 456.

Symbol

CAD

NCBI Official Name

Carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase

NCBI Organism

Homo sapiens

Background Reference 01

Iwahana H., Biochem. Biophys. Res. Commun. 219:249-255 (1996) .

Background Reference 02

Davidson J.N., DNA Cell Biol. 9:667-676 (1990) .

Background Reference 03

Olsen J.V., Cell 127:635-648 (2006) .

Other Product Names

PYR1, CAD protein, EC 2.1.3.2, EC 3.5.2.3

Tested Applications

IHC

Protein ID

50403731

Physical Properties

Liquid

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