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C/EBP-alpha (phospho Thr230) Antibody

Product Specifications

Background

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

NCBI Gene ID

1050

Swiss Prot

P49715

Accession Number

NP_004355.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

C/EBP-alpha (phospho Thr230) antibody was raised against a peptide sequence around phosphorylation site of threonine 230 (P-P-T (p) -P-V) derived from Human C/EBP-alpha.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

42 kDa

Fragment

IgG

Specificity

The Antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at Thr230.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CEBPA

NCBI Official Name

CCAAT/enhancer binding protein (C/EBP), alpha

NCBI Organism

Homo sapiens

Background Reference 01

Xanthopoulos K.G. Biochem. Biophys. Res. Commun. 215:106-113 (1995)

Background Reference 02

Schalwijk J. Biol. Chem. Hoppe-Seyler 378:373-379 (1997)

Background Reference 03

SeattleSNPs variation discovery resource Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases

Other Product Names

CCAAT/enhancer-binding protein alpha C/EBP alpha CEBPA

Tested Applications

WB

Protein ID

28872794

Physical Properties

Liquid

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