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AML1 (phospho Ser435) Antibody

Product Specifications

Background

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.

NCBI Gene ID

861

Swiss Prot

Q01196

Accession Number

NP_001001890.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

AML1 (phospho Ser435) antibody was raised against a peptide sequence around phosphorylation site of Serine 435 (S-N-S (p) -P-T) derived from Human AML1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

53 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

RUNX1

NCBI Official Name

Runt-related transcription factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434 (1991) .

Background Reference 02

Sacchi N., Genes Chromosomes Cancer 11:226-236 (1994) .

Background Reference 03

Nucifora G., Blood 81:2728-2734 (1993) .

Other Product Names

Acute myeloid leukemia 1 protein, CBFA2, CBFA2, alpha 2 subunit, RUNX1

Tested Applications

WB

Protein ID

223459612

Physical Properties

Liquid

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